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Details




NbTI03D01

Phenotype Description
Severely stunted plants, mottled, crinkled, white green leaves
Annotation
Solyc10g081530.1.1;evidence_code:10F1H1E1IEG genomic_reference:SL2.50ch10 gene_region:61897645-61902538 transcript_region:SL2.50ch10:61897645..61902538- functional_description:"V-type proton ATPase subunit d 1 (AHRD V1 ***- D3PHZ2_9MAXI); contains Interpro domain(s) IPR016727 ATPase, V0 complex, subunit D "
ref|XP_009765131.1|;PREDICTED: V-type proton ATPase subunit d2 [Nicotiana sylvestris]ref|XP_009765132.1| PREDICTED: V-type proton ATPase subunit d2 [Nicotiana sylvestris]
NbS00060047g0005.1;protein AED:0.17 eAED:0.17 QI:256|1|1|1|1|1|10|414|351; (*GB) gi|357501685|ref|XP_003621131.1| (e_value=0.0) V-type proton ATPase subunit d2 [Medicago truncatula];; (*SWP) sp|Q9LHA4|VA0D2_ARATH (e_value=0.0) V-type proton ATPase subunit d2 OS=Arabidopsis thaliana GN=VHA-D2 PE=2 SV=1;; (*TAIR) AT3G28715.1 (e_value=0.0) | Symbols: | ATPase, V0/A0 complex, subunit C/D | chr3:10778025-10780350 FORWARD LENGTH=351;; (*ITAG) Solyc10g081530.1.1 (e_value=0.0) evidence_code:10F1H1E1IEG genomic_reference:SL2.40ch10 gene_region:61897645-61902538 transcript_region:SL2.40ch10:61897645..61902538- functional_description:"V-type proton ATPase subunit d 1 (AHRD V1 ***- D3PHZ2_9MAXI); contains Interpro domain(s) IPR016727 ATPase, V0 complex, subunit D ";
AT3G28710.1;| Symbols: | ATPase, V0/A0 complex, subunit C/D | chr3:10773144-10775594 REVERSE LENGTH=351
GO ID (Nr annotation)
GO:0033179 GO:0015078 GO:0015991
GO ID (Arabidopsis annotation)
GO:0033179 GO:0015078 GO:0015991
GO ID (Tomato annotation)
GO:0033179 GO:0015078 GO:0015991
GO ID (Niben annotation)
GO:0033179 GO:0015078 GO:0015991
Sequence
>NbTI03D01
GTGATGACCGTAGGAAGTTGTACTCTAATTTTGAGCTTGCTTTATCCTTATGGTCATGAGGAACTTGCCATCTGCGAAGATATAGATCAGGTCCGTGGTG
TCATGGAGAAGTACCCTCCTTATCAATCCATATTCTCTAAGTTAGCTTATGGTGAGAGCCAGATGCTCGACAAGGGTTTTTATGAAGAGGAAGTCAAACG
CCTTTGCCTGGCATTTGAACAACAGTTCCACTATGGTGTATTTTTTGCATACGTAAGGCTGAGAGAGCAGGAGATCAGGAACTTAATGTGGATATCTGAA
TGTGTGGCTCAGAACCAGAAATCCAGAGTCCATGACAGTGTTGTTTTATTATTTTAATGATCTGGTTATTCAGATTATGTGCTGTGCATACTCTTTGTAT
ATCTGAGTTGCACTGCAGTTTGTCGATTGATATGAGTTATTCTCGGGGTCAAGGCTAACCTCAGCCGTGTTACGGGGGTCATGCTTCGAAAAGTTATCCT
CCAGGAAACTTGCCAGTCCTCCTGCGTGCTTCCTGAGTTGATTATTGTGAGTTGCAAAGAGATATTAGCCTGATCGTTGTGTATCTTCTCTAATAAATTC
AGTGTATGCATGGTTGAATATCATCTGGAATGATCCCCGGATTGATATGTATCTGAAATTCTCTATTTTTTAAACTTGATCACAATAAAATTTCTGC
More analysis about the clones: Nicotiana benthamiana resources at Boys Thomson Institute
To find off target genes: siRNA scan
To predict efficiency of siRNA: pssRNAit

2-1:3A1-3D12[7-12-12]//3D1..JPG