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Details




NbTI03E01

Phenotype Description
Plants were similar to vector control plants
Annotation
Solyc03g116290.1.1;evidence_code:10F0H0E0IEG genomic_reference:SL2.50ch03 gene_region:59822418-59824195 transcript_region:SL2.50ch03:59822418..59824195+ functional_description:"Gibberellin 2-beta-dioxygenase 2 (AHRD V1 **-* G2OX2_ARATH); contains Interpro domain(s) IPR005123 Oxoglutarate and iron-dependent oxygenase "
ref|XP_012469551.1|;PREDICTED: flavonol synthase/flavanone 3-hydroxylase-like [Gossypium raimondii]gb|KJB06470.1| hypothetical protein B456_001G072100 [Gossypium raimondii]
NbS00000948g0004.1;protein AED:0.06 eAED:0.08 QI:0|0.66|0.25|1|0.33|0.25|4|0|345; (*GB) gi|224119092|ref|XP_002317983.1| (e_value=2e-103) predicted protein [Populus trichocarpa];; (*SWP) sp|Q96330|FLS1_ARATH (e_value=3e-32) Flavonol synthase/flavanone 3-hydroxylase OS=Arabidopsis thaliana GN=FLS1 PE=1 SV=1;; (*TAIR) AT5G08640.2 (e_value=2e-33) | Symbols: FLS1 | flavonol synthase 1 | chr5:2804009-2805175 FORWARD LENGTH=336;; (*ITAG) Solyc03g116290.1.1 (e_value=5e-98) evidence_code:10F0H0E0IEG genomic_reference:SL2.40ch03 gene_region:59822418-59824195 transcript_region:SL2.40ch03:59822418..59824195+ functional_description:"Gibberellin 2-beta-dioxygenase 2 (AHRD V1 **-* G2OX2_ARATH); contains Interpro domain(s) IPR005123 Oxoglutarate and iron-dependent oxygenase ";
AT3G19010.1;| Symbols: | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | chr3:6556306-6557862 REVERSE LENGTH=349
GO ID (Nr annotation)
GO:0051213
GO ID (Arabidopsis annotation)
GO:0043231 GO:0044425 GO:0005488 GO:0016491 GO:0001819 GO:0002376 GO:0002682 GO:0006355 GO:0006915 GO:0006952 GO:0007165 GO:0007275 GO:0010557 GO:0010605 GO:0010628 GO:0031328 GO:0043067 GO:0043410 GO:0044237 GO:0044710 GO:0045595 GO:0045860 GO:0048523 GO:0050708 GO:0051050 GO:0051128 GO:0051173 GO:0051179 GO:0051707 GO:0065008 GO:0071310 GO:0071704 GO:2000026
GO ID (Tomato annotation)
GO ID (Niben annotation)
GO:0001669 GO:0005634 GO:0005783 GO:0005886 GO:0031090 GO:0044421 GO:0044425 GO:0045177 GO:0001786 GO:0004175 GO:0004744 GO:0005126 GO:0031210 GO:0046872 GO:0052884 GO:0052885 GO:1901612 GO:0002376 GO:0002675 GO:0007584 GO:0007601 GO:0008284 GO:0015992 GO:0042572 GO:0043065
Sequence
>NbTI03E01
TTGATCTCTCAACTTTCTTCTTGAATGATGAAGATCAAGAGGGCAAGAAGAAGGTTACTGAAATAATCAAACAAGCCTGCTCGGAGTACGGCTTATTTCA
AGTGGTAAACCATGGCGTACCCATAGATTTCATTCACCGAGCTATGGAGGTGTGCAAAGAATTCTTTGATCACCATATGTGGAGAAAGTCAAAGTTTACT
CTGAATCAGATGCTCCACTTCCTTTTCCTCATGGATATCGCAAGAACAAAGCCGATAAGAATGAATATTTGATCATGTCGCCTCCTGGAACCACTGGAAA
TGTTTTCCCTACCAATCCACCAAAATACCAGCTTATTATGGAAAAATTGTTTAATAAATTTTCGGAGACGTGCCAACTCTTGGAGGGCATTCTGAATGAT
TCTCTCGGTCTCCCTCCCAACTGCCTGAAAGATTATAACAACGATAGATGTGCGGATCTTTTGTTGAGTTGTCGTTACTTTCCAGCAACTGAAATGGAAG
ATAATGGTGCTAGCTCACACCAGGACACCTGTTGTTTTGGGCCTCATATTTCAGGATGATGCTGGAGGTCTCGAAGTTCTCATCGATGGGGGAATGGATA
CCTGTGACTCCAATTGAGGGGGCCTTGGTCTTCCATATATGCGATGTTCTTCAGGTGCTAACCAACGACA
More analysis about the clones: Nicotiana benthamiana resources at Boys Thomson Institute
To find off target genes: siRNA scan
To predict efficiency of siRNA: pssRNAit

2-1:3E1-3H12[7-19-12]//3E1..JPG