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Details




NbTI03E03

Phenotype Description
Green leaves
Annotation
Solyc03g117650.2.1;genomic_reference:SL2.50ch03 gene_region:60787314-60791258 transcript_region:SL2.50ch03:60787314..60791258+ functional_description:"Glycosylphosphatidylinositol anchor biosynthesis protein 11 (AHRD V1 ***- B4FCF2_MAIZE); contains Interpro domain(s) IPR009580 GPI biosynthesis protein Pig-F "
ref|XP_009783858.1|;PREDICTED: glycosylphosphatidylinositol anchor biosynthesis protein 11 [Nicotiana sylvestris]
NbS00032073g0009.1;protein AED:0.34 eAED:0.34 QI:30|1|1|1|1|1|8|272|233; (*GB) gi|224072751|ref|XP_002303863.1| (e_value=2e-96) predicted protein [Populus trichocarpa];; (*SWP) sp|O09101|PIGF_MOUSE (e_value=5e-19) Phosphatidylinositol-glycan biosynthesis class F protein OS=Mus musculus GN=Pigf PE=1 SV=1;; (*TAIR) AT1G16040.1 (e_value=1e-67) | Symbols: | FUNCTIONS IN: molecular_function unknown; INVOLVED IN: GPI anchor biosynthetic process; LOCATED IN: integral to membrane, endoplasmic reticulum membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: GPI biosynthesis protein Pig-F (InterPro:IPR009580); Has 280 Blast hits to 280 proteins in 133 species: Archae - 0; Bacteria - 0; Metazoa - 113; Fungi - 111; Plants - 44; Viruses - 0; Other Eukaryotes - 12 (source: NCBI BLink). | chr1:5504747-5506456 FORWARD LENGTH=226;; (*ITAG) Solyc03g117650.2.1 (e_value=5e-120) genomic_reference:SL2.40ch03 gene_region:60787314-60791258 transcript_region:SL2.40ch03:60787314..60791258+ functional_description:"Glycosylphosphatidylinositol anchor biosynthesis protein 11 (AHRD V1 ***- B4FCF2_MAIZE); contains Interpro domain(s) IPR009580 GPI biosynthesis protein Pig-F ";
AT1G16040.1;| Symbols: | FUNCTIONS IN: molecular_function unknown; INVOLVED IN: GPI anchor biosynthetic process; LOCATED IN: integral to membrane, endoplasmic reticulum membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: GPI biosynthesis protein Pig-F (InterPro:IPR009580); Has 280 Blast hits to 280 proteins in 133 species: Archae - 0; Bacteria - 0; Metazoa - 113; Fungi - 111; Plants - 44; Viruses - 0; Other Eukaryotes - 12 (source: NCBI BLink). | chr1:5504747-5506456 FORWARD LENGTH=226
GO ID (Nr annotation)
GO:0005789 GO:0016021 GO:0006506
GO ID (Arabidopsis annotation)
GO:0005789 GO:0016021 GO:0006506
GO ID (Tomato annotation)
GO:0005789 GO:0016021 GO:0006506
GO ID (Niben annotation)
GO:0005789 GO:0016021 GO:0006506
Sequence
>NbTI03E03
ACGCCGTCGATCTCATCACTCATCCATCGCAAACGCTTCGTCTGATTTCTGTGTTTGTGGCTCCAATAGTGATTCTTCTTTACAGTCACCTTCGACACGA
TAGGAGCCAATGCTCTTACGTCAAAGCTATAGGACGAGGCCTACTGGCGCTTCCTGCTGGGGCCGTAGTAAATGCAGCTGGAGCTACTATATTAGGAGCA
CCTGTTGGTTTCGAGTATTTTCCCAAGACCCTTAATTGGTCTCTTCTGATGTCACTGCTCACTTTTGTACCAGCAGCTTGTGTTTTCGGCTCATCATGGA
CTGATTGGCACCGTGTATTTGCTAAAACAAAGACAAATGGGTCCACAGACTGTATGATCTGTCTACCAGCTCATGGAGCTGTTATTGGAGCATGGTTTGG
TGCTTGGCCAATGCCACTGGATTGGGAAAGACCATGGCAGGAATGGCCTATTTGTGTGACTTATGGAGCTATAGCTGGTTATCTCGTGGGGCTTTTGGCA
TCCTTGGGCTGCATTTTCTTCCGTAAGCGCCAGCAGTATCTAAAAGAAGAGTAGAGCCTGGCTTCTGAGAATTAGCCGTGGAAGGCGCTTGATGCTAATA
ATAGTCTTGGGACGAAGTTAGGCCTTTGTTACTCAACTACATAGCTATATGATATCCAATTTTGCTCTCTT
More analysis about the clones: Nicotiana benthamiana resources at Boys Thomson Institute
To find off target genes: siRNA scan
To predict efficiency of siRNA: pssRNAit

2-1:3E1-3H12[7-19-12]//3E3..JPG