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Details




NbTI03H03

Phenotype Description
Stunted, bushy plants, mottled leaves, reduced apical growth
Annotation
Solyc03g059250.2.1;genomic_reference:SL2.50ch03 gene_region:25551514-25557664 transcript_region:SL2.50ch03:25551514..25557664+ functional_description:"Serine/threonine kinase (AHRD V1 *-** C4QNN2_SCHMA); contains Interpro domain(s) IPR002290 Serine/threonine protein kinase "
ref|XP_009792686.1|;PREDICTED: serine/threonine-protein kinase Nek6-like [Nicotiana sylvestris]ref|XP_009792687.1| PREDICTED: serine/threonine-protein kinase Nek6-like [Nicotiana sylvestris]
NbS00015285g0007.1;protein AED:0.17 eAED:0.17 QI:261|1|1|1|0.64|0.6|15|458|645; (*GB) gi|225441728|ref|XP_002277339.1| (e_value=0.0) PREDICTED: serine/threonine-protein kinase Nek6-like [Vitis vinifera];; (*SWP) sp|Q9LT35|NEK6_ARATH (e_value=3e-150) Serine/threonine-protein kinase Nek6 OS=Arabidopsis thaliana GN=NEK6 PE=2 SV=1;; (*TAIR) AT3G20860.1 (e_value=1e-152) | Symbols: ATNEK5, NEK5 | NIMA-related kinase 5 | chr3:7306147-7308434 FORWARD LENGTH=427;; (*ITAG) Solyc03g059250.2.1 (e_value=0.0) genomic_reference:SL2.40ch03 gene_region:25551514-25557664 transcript_region:SL2.40ch03:25551514..25557664+ functional_description:"Serine/threonine kinase (AHRD V1 *-** C4QNN2_SCHMA); contains Interpro domain(s) IPR002290 Serine/threonine protein kinase ";
AT3G20860.1;| Symbols: ATNEK5, NEK5 | NIMA-related kinase 5 | chr3:7306147-7308434 FORWARD LENGTH=427
GO ID (Nr annotation)
GO:0000166 GO:0004674 GO:0006468
GO ID (Arabidopsis annotation)
GO:0005615 GO:0005634 GO:0005737 GO:0003677 GO:0005125 GO:0006997 GO:0007067 GO:0007286 GO:0008284 GO:0030261 GO:0050731 GO:0051301
GO ID (Tomato annotation)
GO:0001669 GO:0005615 GO:0005634 GO:0043234 GO:0070062 GO:0005198 GO:0046872 GO:0009566 GO:0019731 GO:0042628 GO:0048240 GO:0050817 GO:0051291 GO:0090281 GO:1900005 GO:1901318
GO ID (Niben annotation)
GO:0000785 GO:0005654 GO:0005739 GO:0005789 GO:0005829 GO:0005891 GO:0014069 GO:0016323 GO:0031143 GO:0031410 GO:0042383 GO:0043197 GO:0043209 GO:0045211 GO:0070062 GO:0004402 GO:0005096 GO:0005159 GO:0005245 GO:0005506 GO:0008134 GO:0008379 GO:0008392 GO:0008395 GO:0020037 GO:0030331 GO:0031434 GO:0031625 GO:0031691 GO:0031692 GO:0031701 GO:0031762 GO:0031896 GO:0035612 GO:0035615 GO:0043027 GO:0044212 GO:0044325 GO:0045309 GO:0070330 GO:0000187 GO:0002031 GO:0002032 GO:0002092 GO:0002536 GO:0006366 GO:0007188 GO:0007602 GO:0010310 GO:0016567 GO:0019233 GO:0019369 GO:0019430 GO:0030194 GO:0030817 GO:0030820 GO:0031397 GO:0031398 GO:0031665 GO:0032088 GO:0032092 GO:0032496 GO:0032715 GO:0032717 GO:0033138 GO:0034260 GO:0034393 GO:0034765 GO:0035025 GO:0035774 GO:0036276 GO:0042098 GO:0042699 GO:0042744 GO:0043149 GO:0043154 GO:0043161 GO:0043280 GO:0043524 GO:0043547 GO:0045581 GO:0045944 GO:0048538 GO:0048872 GO:0050975 GO:0055114 GO:0070296 GO:0070373 GO:0070374 GO:0070588 GO:0090240 GO:2000378 GO:2001240
Sequence
>NbTI03H03
AATGGTTCAACCTCACTTAAAGATGACATAGAAAAGAAAGGAAACATGGTTGAATATGCTAAACAGATCGATAAAGATGCTCTTCAAGCACTTGATGATA
GGGTTTCACTGCTTAAGGCACTAGCTGCATTAGCTGAGCACAAAAATGACTGGGAAACTCCTACTCAACAAAGAGCTGAAGCTCTGGAATCCCTTTTAGA
GGTTTGTGCACGACTACTTAAGCAAGAAAAAATTGATGAGCTCGCAGGTGTGCTGAAACCATTTGGTGACGATACAATTTCATCTAGAGAGACGGCAATA
TGGTTGACTAAAAGCCTCATGAGTGCACAAAAATTGGCCAAGAGATCGTGATTTTGTGATAGCAAGATTTCCCTTTACTGGAATATTACTGTACAAAGCG
ATTTGTTTGCTTTCTGTGGGTTGACATAGATGTTTAGCTTGACTGTTGTTTGATTTCCTTCTAGATGTTTTGATTTATGGGAAAGCTTACTTTGATTGTA
TTTTTCATCAAAGGAGCTTCTCCGGTGTGTAACATGAGCAACCAAAGTGAGAACTAAGTTATTGTTTTAGGCTTGATGGATTGAGGTCCCCTTAGTTTGA
GTGTACAATTGTAGGACATCTGGTGGAATTGTGAAGATCTTAAACTACTCTTGGCTTGGTTTGTT
More analysis about the clones: Nicotiana benthamiana resources at Boys Thomson Institute
To find off target genes: siRNA scan
To predict efficiency of siRNA: pssRNAit

2-1:3E1-3H12[7-19-12]//3H3..JPG