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Details




NbTI03H10

Phenotype Description
Stunted plants, mottled leaves
Annotation
Solyc08g006670.2.1;genomic_reference:SL2.50ch08 gene_region:1247049-1249937 transcript_region:SL2.50ch08:1247049..1249937- functional_description:"Unknown Protein (AHRD V1)"
ref|XP_009769931.1|;PREDICTED: mediator of RNA polymerase II transcription subunit 30 [Nicotiana sylvestris]
NbS00013085g0013.1;protein AED:0.35 eAED:0.37 QI:0|0.33|0.25|1|1|1|4|0|181; (*GB) gi|225456359|ref|XP_002283991.1| (e_value=5e-41) PREDICTED: uncharacterized protein LOC100264705 [Vitis vinifera];; (*TAIR) AT5G63480.1 (e_value=1e-30) | Symbols: | unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). | chr5:25417317-25418473 FORWARD LENGTH=189;; (*ITAG) Solyc08g006670.2.1 (e_value=2e-61) genomic_reference:SL2.40ch08 gene_region:1247049-1249937 transcript_region:SL2.40ch08:1247049..1249937- functional_description:"Unknown Protein (AHRD V1)";
AT5G63480.1;| Symbols: | unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). | chr5:25417317-25418473 FORWARD LENGTH=189
GO ID (Nr annotation)
GO ID (Arabidopsis annotation)
GO ID (Tomato annotation)
GO ID (Niben annotation)
Sequence
>NbTI03H10
GATTTCGACAACATAGGCAGCATTTTCCAGAAAATGGAGGAAAAAGGCGGCACAATGATAAACCCTAAAACAATACAAGAACTAGCAGTAGAAGGACAAA
AGCATTTAGAAGACACAATAGAAGCAGCACATCAAATTCTCTCCGCCATGAACGACGAACTTTGTAACCCTACACTATGGTCAACTACTCCAAATACGGC
CGCTACTACTGCTAATGCCGTTATGAGTAACGGACAGCAGCAGCATCATTCGAACGGCGGCGGCGACGTTTCGTCGGATAACTCTTCGTCTTCTTCGGCA
TCGGCTCAACAACACTTGGATATTGGGGGCGGTGCCCTTGATGAGTCTCGTCTCCGTTATAAGTCGTCAATTTCTTGCTTGCGTTCTGTTCTAACGGCTA
TTTCTAATTCTCAGAAGCTAGGCAAAAGCATTGGAAGCTGCTTCTGCTAGTGGCTCAGTATCTGCTGCAGATCAAGCTGAAATTGAGCAGTTGGAGGAAC
GCGCCGCTGCGTTAAAAAAGGAACTTGTAGACAAGAACAAGCATCTCAAGCTTCTAATTGATCAGCTTCGAGATCTTCTTTCTGACCTATCAACATGGCA
AAGTCCCTGTTCTACGTGACTCTAATCCTATAACTTCAAGCAGATAGTTAAACAAGAGAGATGTTGGATGACAAAGGAACCGCGTTCAACTCTAAGAAGC
TGCTTTTTTGTTGTACAGAGGTTACTGCTAGATCCAAAATA
More analysis about the clones: Nicotiana benthamiana resources at Boys Thomson Institute
To find off target genes: siRNA scan
To predict efficiency of siRNA: pssRNAit

2-1:3E1-3H12[7-19-12]//3H10..JPG