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Details




NbTI09G05

Phenotype Description
Plants were similar to vector control plants
Annotation
Solyc02g069070.2.1;genomic_reference:SL2.50ch02 gene_region:33618808-33621536 transcript_region:SL2.50ch02:33618808..33621536- functional_description:"Ribonuclease H2 subunit C (AHRD V1 ***- C0H7J5_SALSA); contains Interpro domain(s) IPR013924 Ribonuclease H2, subunit C "
ref|XP_009787298.1|;PREDICTED: ribonuclease H2 subunit C [Nicotiana sylvestris]
NbS00053497g0005.1;protein AED:0.14 eAED:0.14 QI:166|1|1|1|0.66|0.75|4|544|167; (*GB) gi|186506604|ref|NP_181476.2| (e_value=2e-59) ribonuclease H2 subunit C [Arabidopsis thaliana];; (*TAIR) AT2G39440.1 (e_value=4e-62) | Symbols: | CONTAINS InterPro DOMAIN/s: Ribonuclease H2, subunit C (InterPro:IPR013924); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). | chr2:16468220-16469113 REVERSE LENGTH=162;; (*ITAG) Solyc02g069070.2.1 (e_value=4e-104) genomic_reference:SL2.40ch02 gene_region:33618808-33621536 transcript_region:SL2.40ch02:33618808..33621536- functional_description:"Ribonuclease H2 subunit C (AHRD V1 ***- C0H7J5_SALSA); contains Interpro domain(s) IPR013924 Ribonuclease H2, subunit C ";
AT2G39440.1;| Symbols: | CONTAINS InterPro DOMAIN/s: Ribonuclease H2, subunit C (InterPro:IPR013924); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). | chr2:16468220-16469113 REVERSE LENGTH=162
GO ID (Nr annotation)
GO:0005634 GO:0032299 GO:0004523 GO:0006401 GO:0090502
GO ID (Arabidopsis annotation)
GO ID (Tomato annotation)
GO ID (Niben annotation)
Sequence
>NbTI09G05
AGAAACACCGGGGGAATTTCAGGCACAATTGATTTAAACGGCAATCTGCCGCCGGGGAATGTCGTGGATCTCACCGGGAAAGTGCACCAGCTGCCATGCT
GTATTAAGTTCAATGGTCCATCTGATGTTTCACAGTACTTCAAACCAAAACMCACTGGTGTAATTGTTGACGGGTTAAATGTGGAGGAAGCTCATTTTAG
AGGAAGGAAGCTTCTGGGAACGACTGTTGCAATTCCTCATGGCTACTCTGGGTTTGTCTTGGGAAAGAAAATGCCTGATGAGAAAAGAAAAAGGTCTGTG
GAAGATTCAAGTTGCTGGGAGATGAAGGCGAAGTTCCAAAACATAACATCGTGGAATCATGATTCTCTTCCCTCGGAAAATGATTCTTTCTTGCGTGCCT
TCCACCTATTTTCTGTTACAACAGCACTGCACCAACCAGTCAGCATTGAAGAACTGGAAGCTGCAGCAATTGATCAAGAGTTAAGAGTTATGAACTGATC
AAGATGCTATGTAGATAACGTCCACAAGGAGCAGTACATTTCGAGCTCGTTTATGCTCTTCGTCAGTTAATGGAAGCTTTCTTCATTATATATCTATTCA
ATCAGGATTCTGTTTATCCTTCATCTAACATCTATAGTGAGAACATATTAAACACATGAGATTGTGCATTCTCGA
More analysis about the clones: Nicotiana benthamiana resources at Boys Thomson Institute
To find off target genes: siRNA scan
To predict efficiency of siRNA: pssRNAit

1-1:9E1-9H12[10-31-12]//9G5.JPG