Noble Research Institute, LLC   Noble Research Institute | VIGS Database  
      VIGS Phenomics and Functional Genomics Database  
Details




NbTI09H08

Phenotype Description
Plants were similar to vector control plants
Annotation
Solyc11g045180.1.1;evidence_code:10F0H1E1IEG genomic_reference:SL2.50ch11 gene_region:36898345-36899171 transcript_region:SL2.50ch11:36898345..36899171- functional_description:"ATP-dependent RNA helicase (AHRD V1 *-*- D3BPQ9_POLPA); contains Interpro domain(s) IPR001650 DNA/RNA helicase, C-terminal "
ref|XP_009766118.1|;PREDICTED: DEAD-box ATP-dependent RNA helicase 50 isoform X2 [Nicotiana sylvestris]
NbS00022637g0010.1;protein AED:0.37 eAED:0.41 QI:0|0.5|0.42|0.85|0.83|0.57|7|849|329; (*GB) gi|297736840|emb|CBI26041.3| (e_value=2e-145) unnamed protein product [Vitis vinifera];; (*SWP) sp|Q8GUG7|RH50_ARATH (e_value=2e-137) DEAD-box ATP-dependent RNA helicase 50 OS=Arabidopsis thaliana GN=RH50 PE=2 SV=2;; (*TAIR) AT3G06980.1 (e_value=2e-138) | Symbols: | DEA(D/H)-box RNA helicase family protein | chr3:2201531-2204662 FORWARD LENGTH=781;; (*ITAG) Solyc11g045180.1.1 (e_value=2e-95) evidence_code:10F0H1E1IEG genomic_reference:SL2.40ch11 gene_region:36898345-36899171 transcript_region:SL2.40ch11:36898345..36899171- functional_description:"ATP-dependent RNA helicase (AHRD V1 *-*- D3BPQ9_POLPA); contains Interpro domain(s) IPR001650 DNA/RNA helicase, C-terminal ";
AT3G06980.1;| Symbols: | DEA(D/H)-box RNA helicase family protein | chr3:2201531-2204662 FORWARD LENGTH=781
GO ID (Nr annotation)
GO:0003676 GO:0003916 GO:0004004 GO:0010501
GO ID (Arabidopsis annotation)
GO:0016020 GO:0044464 GO:0008233 GO:0044699
GO ID (Tomato annotation)
GO ID (Niben annotation)
GO:0005622 GO:0005488 GO:0044763
Sequence
>NbTI09H08
AGTCCGGACACAGCTTTTATCAATAAAAAGAATGCCCTACTTCATCTTGTGGAGGATAGCMCAGTTCCAAAAACAATTGTCTTCTGTAACAAGATTGATA
GCTGCAGAAAAGTTGAGAATGCATTAAAGCGCTTTGACAGGAAAGGATTCTCCATAAAAATTTTACCCTTCCATGCTGCACTGGACCAAGGAAGGAGACT
TGCAAACATGGAGGAGTTTCGCCGCTCAAGGATGGAGAATGTTTCTTTGTTCTTGGTGTGCACCGACAGAGCATCCCGAGGTATCGACTTTGAAGGTGTA
GACCATGTGGTCCTCTTTGATTATCCTCGAGACMCTAGCGAATATGTACGTCGTGTTGGAAGAACTGCCAGGGGTGCTGGTGGAAAAGGAAAGGCATTCA
TATTTGCTGTTGGGAAGCAAGTTTCTCTTGCTCGAAGGATAATGGAGAGAAACAAGAAAGGCCACMCAGTCCATGATGTGCCGTCAATATTAACTTAGCA
TCCTGCAAAAGGGCTCATGGAACCTGGAAAACAGGGCAGTGTGATGCTGGTGCGCTTAGAAGGCCATTGAGAAGAAGCAAAAGAATTAGAAACCATCACA
GGGGCTCACATATTATACTTGGGATCCAGGTTAAGGGCTTGGCTCTGTCAAAAGA
More analysis about the clones: Nicotiana benthamiana resources at Boys Thomson Institute
To find off target genes: siRNA scan
To predict efficiency of siRNA: pssRNAit

1-1:9E1-9H12[10-31-12]//9H8.JPG